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Rat EPO(Erythropoietin) ELISA Kit

Cat.No.:E-EL-R0007
Reactivity: Rat

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48T
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat EPO in samples. No significant cross-reactivity or interference between Rat EPO and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat EPO concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat EPO. You can calculate the concentration of Rat EPO in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat EPO were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat EPO were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	221.70	514.30	1746.70	219.90	557.70	1706.10
Standard deviation	11.30	30.90	76.90	14.70	25.70	83.60
CV (%)	5.10	6.01	4.40	6.68	4.61	4.90

Recovery

The recovery of Rat EPO spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-101	95
EDTA plasma (n=8)	95-111	101
Cell culture media (n=8)	88-103	95

Linearity

Samples were spiked with high concentrations of Rat EPO and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	EP, MVCD2, Epoetin, Hematopoietin, Hemopoietin
Research Area	Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

CLINICAL SCIENCE (2021) IF: 6.124
Selective HIF stabilization alleviates hepatocellular steatosis and ballooning in a rodent model of 70% liver resection

DOI: 10.1042/CS20210183

PMID: 34550341
Sample: plasma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019) IF: 4.183
Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin

DOI: 10.3390/ijms20204985

PMID: 31600973
Sample: Cell culture supernatant
Journal of Diabetes Research (2019) IF: 3.04
The Haematological Effects of Oleanolic Acid in Streptozotocin-Induced Diabetic Rats: Effects on Selected Markers

DOI: 10.1155/2019/6753541

PMID: 31828165
Sample: Plasma,Tissue homogenate
JOURNAL OF ETHNOPHARMACOLOGY (2018) IF: 3.115
Telfairia occidentalis Hook.f. - associated haematopoietic effect is mediated by cytokines but independent of testosterone: A preliminary report

DOI: 10.1016/j.jep.2018.01.018

PMID: 29353002
Sample: Plasma
Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168
Heamanetic Effects of a Dioxidovanadium(V) Complex in STZ-Induced Diabetic Male Sprague Dawley Rats

DOI: 10.2147/DMSO.S214726

PMID: 34707382
Sample: plasma
JOURNAL OF INORGANIC BIOCHEMISTRY (2020) IF: 3.212
In anemia zinc is recruited from bone and plasma to produce new red blood cells

DOI: 10.1016/j.jinorgbio.2020.111172

PMID: 32659518
Sample: Plasma
Frontiers in Veterinary Science (2022) IF: 3.471
Ameliorative Effects of Bovine Lactoferrin on Benzene-Induced Hematotoxicity in Albino Rats

DOI: 10.3389/fvets.2022.907580

PMID: 35812844
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
The effects of Momordica balsamina methanolic extract on haematological function in streptozotocin-induced diabetic rats: Effects on selected markers

DOI: 10.1016/j.biopha.2019.108925

Sample: plasma

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