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Rat MAU(Microalbuminuria) ELISA Kit

Cat.No.:E-EL-R0025
Reactivity: Rat

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96T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 μg/mL
Sensitivity	0.94 μg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat MAU in samples. No significant cross-reactivity or interference between Rat MAU and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat MAU concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MAU. You can calculate the concentration of Rat MAU in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MAU were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MAU were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (μg/mL)	5.10	8.60	47.90	4.90	8.20	49.20
Standard deviation	0.30	0.40	1.70	0.30	0.40	1.50
CV (%)	5.88	4.65	3.55	6.12	4.88	3.05

Recovery

The recovery of Rat MAU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-107	98
EDTA plasma (n=8)	91-103	98
Cell culture media (n=8)	90-104	98

Linearity

Samples were spiked with high concentrations of Rat MAU and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	UMA
Research Area	Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
Empagliflozin reduces kidney fibrosis and improves kidney function by alternative macrophage activation in rats with 5/6-nephrectomy

DOI: 10.1016/j.biopha.2022.113947

PMID: 36411661
Sample: urine
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2021) IF: 4.076
Ezetimibe attenuates experimental diabetes and renal pathologies via targeting the advanced glycation, oxidative stress and AGE-RAGE signalling in rats

DOI: 10.1080/13813455.2021.1874996

PMID: 33508970
Sample: Plasma
PHYTOMEDICINE (2019) IF: 4.18
Cyclocarya paliurus triterpenic acids fraction attenuates kidney injury via AMPK-mTOR-regulated autophagy pathway in diabetic rats

DOI: 10.1016/j.phymed.2019.153060

PMID: 31401495
Sample: Urine
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322
Renoprotective effects of oleanolic acid and its possible mechanisms in rats with diabetic kidney disease

DOI: 10.1016/j.bbrc.2022.10.074

PMID: 36332469
Sample: Urine
Toxicology Research (2022) IF: 3.524
Cerium oxide nanoparticles attenuate the renal injury induced by cadmium chloride via improvement of the NBN and Nrf2 gene expressions in rats

DOI: 10.1093/toxres/tfac009

PMID: 35510236
Sample: Urine
Experimental and Therapeutic Medicine (2018) IF: 1.41
Tangshen formula improves inflammation in renal tissue of diabetic nephropathy through SIRT1/NF-κB pathway

DOI: 10.3892/etm.2017.5621

Sample: Urine
BIOLOGICAL & PHARMACEUTICAL BULLETIN (2018) IF: 1.694
Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

DOI: 10.1248/bpb.b17-00930

PMID: 30068867
Sample: Plasma

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