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Human CA9 (Carbonic Anhydrase IX) CLIA Kit
Synonyms:
Product Introduction
PrincipleDescriptionComponentsProcedureReference

The micro CLIA plate provided in this kit  has been pre-coated with an antibody specific to Human CA9. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the  specific antibody. Then a biotinylated detection antibody specific for Human CA9 and Avidin-Horseradish  Peroxidase (HRP) conjugate is added to each micro plate well successively and  incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA9, biotinylated detection  antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence  immunoassay analyzer. The RLU value is positively associated with the  concentration of Human CA9. You can calculate the  concentration of Human CA9 in the samples by  comparing the RLU value of the samples to the standard curve.


  Full Name   Human CA9 (Carbonic Anhydrase IX) CLIA Kit
  Product ID   E-CL-H0015   Organism   Human
  Package   96T   Price   Inquire
  Detection Range   15.625~1000pg/mL   Sensitivity   9.375pg/mL
  Item   Specifications   Storage
  Micro CLIA Plate   8 wells ×12   4℃
  Reference Standard   2 vials   4℃
  Reference Standard & Sample Diluent   1vial 20mL   4℃
  Concentrated Biotinylated Detection Ab   1vial 120μL   4℃
  Biotinytated Detection Ab Diluent   1vial 10mL   4℃
  Concentrated HRP Conjugate   1vial 120μL   4℃(shading light)
  HRP Conjugate Diluent   1vial 10mL   4℃
  Concentrated Wash Buffer (25×)   1vial 30mL   4℃
  Substrate Reagent A   1vial 5mL   4℃(shading light)
  Substrate Reagent B   1vial 5mL   4℃(shading light)
  Plate Sealer   5 pieces
  Manual   1 copy
  Desiccant   1 pack
  Certificate of Analysis   1 copy

1. Add 100μL standard or sample to each well. Incubate 90 mintues at 37℃

    2. Add 100μL Biotinylated Detection Ab. Incubate 1 hour at 37℃

    3. Aspirate and wash 3 times

    4. Add 100μL HRP Conjugate. Incubate 30 minutes at 37℃

    5. Aspirate and wash 5 times

    6. Add 100μL Substrate Reagent. Incubate 5 minutes at 37℃

    7. Read immediately after the substrate  reaction time

    8. Calculation of results

      If you need the manual for more details, you can click here
  Region   Name   Email
  North America   John
  Alice
  john@elabscience.com
  alice@elabscience.com
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  Japan & Taiwan   Yumi   yumi.elabscience@gmail.com
  South Asia & Australia   Hanny   hanny@elabscience.com
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  Southern Europe & Africa   Lucy   lucy@elabscience.com
  Western & Middle Europe   Perry   Perry.elabscience@gmail.com

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