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Biotin quantitative determination kit
This kit uses Competitive-method. The microtiter plate provided in this kit has been pre-coated with Biotin. During the reaction, Biotin in the sample or standard competes with a fixed amount of Biotin on the solid phase supporter for sites on the Avidin-HRP specific to Biotin. Excess Avidin-HRP and unbound sample or standard are washed from the plate. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.
| Full Name|| Biotin quantitative determination kit |
| Product ID|| EBD0325|
| Package|| 96T|| Price|| $320|
| Detection Range|| 0.313~20ng/mL|| Sensitivity|| 0.188ng/mL|
| Item|| Specifications|| Storage|
| Micro ELISA Plate|| 8 wells ×12 strips|| 4℃|
| Reference Standard|| 2 vials|| 4℃|
| Reference Standard & Sample Diluent|| 1vial 20mL|| 4℃|
| Concentrated Avidin-HRP || 1vial 120μL|| 4℃(shading light)|
| HRP Conjugate Diluent || 1vial 10mL|| 4℃|
| Concentrated Wash Buffer (25×) || 1vial 30mL|| 4℃|
| Substrate Reagent|| 1vial 10mL|| 4℃(shading light)|
| Stop Solution|| 1vial 10mL|| 4℃|
| Plate Sealer|| 5 pieces|
| Manual|| 1 copy|
| Desiccant|| 1 pack|
| Certificate of Analysis|| 1 copy|
1. Add 50μL standard or sample to each well.
2. Immediately add 50μL Avidin-HRP to each well.
3. Incubate for 30 minutes at 37℃.
4. Aspirate and wash 5 times.
5. Add 90μL Substrate Reagent. Incubate 15 minutes at 37℃.
6. Add 50μL Stop Solution. Read at 450nm immediately.
7. Calculation of results.
Economical:low-cost; can be used for batchtesting;
Efficient:only one hour is needed for whole test;
simple sample preparation: no complicated sample processing is needed before experiment;
High sensitivity:the sensitivity reaches 200pg/mL ;
Strong stability: valid for 1 year at low temperature;
| If you need the manual for more details, you can click here |
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