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TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as ready-to-use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO.
One-component TMB Substrate yields a blue color (Amax = 370nm and 652nm) when reacted with peroxidase in microwell applications. The product changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.
Economical: Cost less compared with similar products;
Convenience: mono-component, ready-to-use;
High sensitivity: Save the dosage of antibody;
Strong stability: Valid for 2 years at 2~8℃;
Stable signal: The signal is continuous steady after adding stop solution;
Safe: Non carcinogenic
One-component TMB Substrate is stable for 2 years stored at 2°C - 8°C. Shading light.
Peroxidase-based enzyme immunoassays ELISA is recommended.
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