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The FITC Labeling Kit of Elabscience offering a collection of reagents required for FITC labeling, is designed to label antibody with amidogen(NH2-). The specially treated FITC in the kit can be useddirectly. The reagents are enough for approximately 3 labeling reactions each containing 0.1-2mg of antibody or other protein. Each kit includes 3 Filtration tubes for desalination of antibody labeling without the need for dialysis.The whole procedure is simple and can be completed in 100min in proficient operation.
■ All-inclusive： The kit provides all the reagents required for FITC labeling.
■ Quick： The whole procedure takes only 100min.
■ Convenient：Desalination can be achieved by Filtration tube, sparing the need for dialysis or gel filtration.
■ Flexible：The procedure can be easily adapted to both small and large scales, with 0.2-2mg of protein labeled each time.
■ Perfect results：The kit has been optimized to determine the optimum labeling ratio of FITC to antibody, lowering the possibility of protein inactivation resulted from excess FITC labeling.
|Reactive Group||Amine group (-NH2)|
|Storage||The kit is stable for 1 year at 2-8°C before opening.|
Protein bonds covalently to FITC by thiourea connection as free amino groups of lysine residues in the protein molecule can react with nucleophilic FITC.
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