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■ Quick and easy：Double reagent, direct operation with 96 wells plate.
■ Micro-amount for sample： Sample amount needed is 1/10 of hydroxylamine method.
■ High sensitivity：Detection line is 0.5 U/ml, five times of hydroxylamine method, 200 times of pyrogallol method.
■ Stability：Intra-assay CV = 5.50%, inter-assay CV = 3.32%.
■ Perfect results：CV = 1.2%.
■ Recovery test：X = 102.3%.
■ Less affected by external factors：Less interference factors, good repeatability and specificity, not affected by substances like SOD.
■ Wide testing range：Animal blood, tissue, body fluids, perfusion liquid, culture cell, bacteria, plant tissue, aquatic products and cosmetics, health products and so on.
|Full Name||Superoxide Dismutase (SOD) assay kit|
|Storage||The kit is stable for 3 months at 2-8°C before opening.|
Superoxide dismutase plays a vital role in body balancing status of oxidation and antioxidation. This enzyme can remove superoxide anion free radical , protect cells from damage. The activity of SOD was measured by WST-1 method in this kit. Water-soluble tetrazolium, the sodium salt of 4-[3-(4iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate(WST-1), was used as a detector of superoxide radical generated by xanthine oxidase and hypoxanthine. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
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