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■ The operation is simple：single-agent operation, it can measure above 50 samples in 20 minutes..
■ High sensitivity： the detection range is 0.2-1.3mg/ml, it’s about four times higher than Lowry method.
■ Good reproducibility：Intra-assay CV = 103%, Inter-assay CV = 5.34%.
■ Recovery test：X = 101.8%.
■ Less affected by external factors：ess interference factors, the interference in Lowry method do not interfere this assay method, like K +, Na +, Mg +, Tris buffer, sucrose, glycerol, mercaptoethanol, EDTA and others.
■ Good stability：it can be stored for six months at 2 - 8℃, good color stability.
|Full Name||Total protein quantitative assay kit (With standard: Coomassie brilliant blue method)|
|Package||100 tubes /96 samples|
|Method||Coomassie brilliant blue method|
|Storage||The kit is stable for 6 months at 2-8°C before opening.|
Accurately quantifying total protein concentration is the one of commonly used and basic analysis methods in research of Biochemistry. When add the brilliant blue reagent with brownish-red coomassie to protein standards or samples, the anion of Coomassie brilliant blue G-250 dye bind to the -NH3+ of protein, the solution turns blue. Measure the absorbance of solution to determine the concentration of protein.
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