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H+, K+-ATPase assay kit
Product Introduction


Advantages:

 Quick and easy:it can measure 100 samples in 50 minutes..

 Micro sample amount: It only need 100μl sample.

 Good reproducibility:CV = 1.7%.

 Recovery test:X = 103.3%.

 Less affected by external factors:less interference factors and good repeatability.

 Wide testing range:It can measure animal tissue, erythrocyte, all sorts of culture cells, aquatic products and so on.


Specification:

Full NameH+, K+-ATPase assay kit
Cat. No.BC0122
Package200 tubes /96 samples
MethodUV colorimetry
StorageThe kit is stable for 6 months at 2-8°C before opening.


Principle:

H+, K+-ATPase has important physiological significance in gastric acid secretion and gastric digestive function. Forte et al first isolated a class of ATPase in bullfrog gastric mucosa that can be specific activated by K + and can't inhibited by ouabain, in the 70's.It is H+、K+-ATPase. H + K + -ATPase located in the cell of Gastric mucosa wall, belong to the second class of proton pump. Through its phosphorylation (E1→E2) and dephosphorylated (E2→E1) to complete the H + /K + electric neutrality transmembrane ion transport, constantly transport  proton within the wall cell to outside the cell membrane to perform acid secretion function.


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