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The Long-arm Biotin Labeling Kit of Elabscience offering a collection of reagents required for biotin labeling, is designed to label antibody with amidogen(NH2-). Long-arm reduces the steric hindrance when several biotinylated molecules combine with the same avidin molecular compound, effectively optimizes the labeling and detection results. The biotin in the kit is sufficient and has been activated for direct use. The reagents are enough for approximately 3 labeling reactions each containing 0.1-2mg of antibody or other protein. Each kit includes 6 Filtration tubes for desalination of antibody labeling without the need for dialysis. The whole procedure is simple and can be completed in 90min in proficient operation.
■ Chemical structure：
■ Molecular Weight：568.
■ Molecular formula：C26H41N5O7S.
■ Spacer arm length：30.5 Å.
■ All-inclusive： The kit provides all the reagents required for biotin labeling
■ Quick： The whole procedure takes only 90min.
■ Convenient：Desalination can be achieved by Filtration tube, sparing the need for dialysis or gel filtration.
■ Flexible：The procedure can be easily adapted to both small and large scales, with 0.1-2mg of protein labeled each time.
■ Perfect results：The kit has been optimized to determine the optimum labeling ratio of biotin to antibody, lowering the possibility of protein inactivation resulted from excess biotin labeling.
|Reactive Group||Amine group (-NH2)|
|Storage||The kit is stable for 1 year at 2-8°C before opening.|
The NH2-Reactive Biotin reacts with the primary amine(N-terminal and the side chain of lysine residue) specifically, forming stable amide bond.
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