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Cat.No.:PKSH030290
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Synonyms | Low affinity immunoglobulin gamma Fc region receptor II-a;IgG Fc receptor II-a;CDw32;Fc-gamma RII-a;Fc-gamma-RIIa;FcRII-a;CD32;FCGR2A;FCG2;FCGR2A1;IGFR2;CD32A;CDw32;Fc gamma RIIA;FCG2;FcGR;FCGR2 |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-Met 210 |
Accession | P12318-1 |
Application | Functional ELISA |
Mol_Mass | 23.6 kDa |
AP_Mol_Mass | 33 kDa |
Tag | C-His-Avi |
Bio_Activity | 1. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Labeling ratio of biotin to protein: 0.1-0.6. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |