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Cat.No.:PKSH030445
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Synonyms | SGRF;IL-23p19;CLMF p40;IL-12 subunit p40;NKSF2;IL-23;IL-23A;IL23P19;P19 |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-Pro189&Met 1-Ser 28 |
Accession | Q9NPF7&NP_002178.2 |
Application | Functional ELISA |
Mol_Mass | 20.1&36.2 kDa |
AP_Mol_Mass | 22&45 kDa |
Tag | C-His |
Bio_Activity | 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml. 3. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml. 4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |