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This kit utilizes the principles of ELISA. It is comprised of microplate coated with Porcine Circovirus, enzyme-conjugated second antibody and other reagents. The reaction mechanism is that envelope antigen combines with PCV-IgG of the sample, then combines with enzyme-labeled anti-swine IgG antibody which forms a compound of "envelope antigen + PCV–IgG + enzyme –labeled anti-swine IgG antibody", adding color agent, developing through enzyme-catalysis reaction. The level of coloration is directly proportional with the quantity of PCV-IgG, and the result will turns out to be positive when it exceed the threshold, which shows the immunation works and natural infection exist.
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