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Diagnostic Kit for chicken Antibodies to Newcastle Disease Virus (ELISA)
Product Introduction
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Intended usePrinciple of the assayMaterials provided with the kitStorage

Chicken newcastle disease also known as Asian fowl plague or pseud-fowl plague is an acute contagious disease caused by newcastle disease virus (NDV).its main features are dyspnea, loose stool, neuropsychiatric disorders and mucosal and seromembranous hemorrhage, etc.This kit can be used to detect the antibody of NDV in chicken serum and evaluate the NDV vaccine immunization status.

This kit is comprised of microplate coated with NDV antigen, enzyme-conjugated second antibody and other reagents. It utilizes the principles of ELISA to detect antibody specific to NDV in chicken serum and plasma. Control serum and diluted serum samples are added into the microplate during experiment. After incubation, if the sample contains antibody specific to NDV, the antibody will bind with the antigen coated in the microplate. The microplate should be washed with wash solution to remove unbound antibody and other components. The enzyme- conjugated second antibody is added into the washed microplate and it will specifically bind with the antibody/antigen complex on the microplate. The microplate should be washed once again to remove unbound enzyme conjugate. Then TMB substrate solution is added into wells to react with enzyme and appears blue in color. The enzyme-substrate reaction is terminated by the addition of the stop solution, the color changes from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm and proportional to the concentration of specific antibody.

  1.   Micro ELISA Plate   96T×1
  2.   Enzyme Conjugate (red cap)   11mL×1
  3.   Sample diluent (blue cap)   50mL×1
  4.   20×Concentrated wash solution   40mL×1
  5.   Chromogen solution A(brown cap)   6mL×1
  6.   Chromogen solution B(black cap)   6mL×1
  7.   Stop solution(yellow cap)   6mL×1
  8.   Positive control(blue cap)   0.5mL×1
  9.   Negative control(blue cap)   0.5mL×1
  10.   Plate Sealer   1
  11.   Instruction   1

The kit is stored at 2~8℃.

      If you need the manual for more details, you can click here
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