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Diagnostic Kit for Antibodies to Avian Influenza Virus (H5N1) (ELISA)
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Intended usePrinciple of the assayMaterials provided with the kitStorage

Avian influenza briefly called avian flu caused by avian influenza virus (AIV) is an acute, highly lethal and contagious disease. It can cause issues in respiratory system or even cause systemic septicemia. Avian influenza is also known as true fowl plague or Europe fowl plague.This kit can be used to detect the antibody of Avian Influenza Virus (H5N1) in chicken serum and evaluate the AIV vaccine immunization status.

This kit is comprised of microplate coated with AIV (H5N1) antigen, enzyme-conjugated second antibody and other reagents. It utilizes the principles of ELISA to detect antibody specific to AIV (H5N1) in chicken serum and plasma. Control serum and diluted serum samples are added into the microplate during experiment. After incubation, if the sample contains antibody specific to AIV (H5N1), the antibody will bind with the antigen coated in the microplate. The microplate should be washed with wash solution to remove unbound antibody and other components. The enzyme- conjugated second antibody is added into the washed microplate and it will specifically bind with the antibody/antigen complex on the microplate. The microplate should be washed once again to remove unbound enzyme conjugate. Then TMB substrate solution is added into wells to react with enzyme and appears blue in color. The enzyme-substrate reaction is terminated by the addition of the stop solution, the color changes from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm and proportional to the concentration of specific antibody.

  1.   Micro ELISA Plate   96T×1
  2.   Enzyme Conjugate (red cap)   11mL×1
  3.   Sample diluent (blue cap)   25mL×1
  4.   20×Concentrated wash solution   25mL×1
  5.   Chromogen solution A(brown cap)   7mL×1
  6.   Chromogen solution B(black cap)   7mL×1
  7.   Stop solution(yellow cap)   7mL×1
  8.   Positive control(blue cap)   0.5mL×1
  9.   Negative control(blue cap)   0.5mL×1
  10.   Plate Sealer   1
  11.   Instruction   1

The kit is stored at 2~8℃. Do not freeze.

      If you need the manual for more details, you can click here
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