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Diagnostic Kit for Antibodies to foot and mouth disease virus type O(ELISA)
Product Introduction
Intended usePrinciple of the assayMaterials provided with the kitStorage

The kit is used to detect the antibody of FMDV in the pig, cattle, sheep serum and evaluate the vaccine immunization status of FMDV.

This kit utilizes the principles of blocking ELISA method. The antigen of FMDV was pre coated on the microplate. Control serum and diluted serum samples are added into the microplate during experiment. After incubation, if the sample contains antibody specific to FMDV, the antibody will bind with the antigen coated in the microplate. The microplate should be washed with wash solution to remove unbound antibody and other components. The enzyme- conjugated VP1 monoclonal antibody is added into the washed microplate, the antibodies in the sample will block the binding of monoclonal antibodies to the antigen on the plate. The microplate should be washed once again to remove unbound enzyme conjugate. Then TMB substrate solution is added into wells to react with enzyme and appears blue in color. The enzyme-substrate reaction is terminated by the addition of the stop solution, the color changes from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

  1.   Micro ELISA Plate   96T×1
  2.   Enzyme Conjugate   11mL×1
  3.   10×Concentrated wash solution   50mL×1
  4.   Color-substrate solution   11mL×1
  5.   Sample diluent   50mL×1
  6.   Stop solution   6mL×1
  7.   Negative control   1mL×1
  8.   Positive control   0.5mL×1
  9.   Plate Sealer   2
  10.   Instruction   1

The kit is stored at 2~8℃.

      If you need the manual for more details, you can click here
  Region   Name   Email
  North America   John
  South America   Gwyneth
  Asia   Britney
  Japan & Taiwan   Yumi
  South Asia & Australia   Hanny
  Eastern & Northern Europe   Cathy
  Southern Europe & Africa   Lucy
  Western & Middle Europe   Perry

Phone: 1-240-252-7368     1-240-252-7376