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Diagnostic Kit for Antibodies to Cattle foot and mouth disease virus type A (ELISA)
Product Introduction
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Intended usePrinciple of the assayMaterials provided with the kitStorage

This kit is used to test the antibody of foot and mouth disease virus (FMDV) type A in bovine serum. It can also be used to evaluate the vaccine immunization status of FMDV type A.

This kit utilizes the principles of indirect ELISA method. The antigen of FMDV was pre coated on the microplate. Control serum and diluted serum samples are added into the microplate during experiment. After incubation, if the sample contains antibody specific to FMDV, the antibody will bind with the antigen coated in the microplate. The microplate should be washed with wash solution to remove unbound antibody and other components. The enzyme-conjugated second antibody is added into the washed microplate, and it will specifically bind with the antibody/antigen complex on the microplate. The microplate should be washed once again to remove unbound enzyme conjugate. Then TMB substrate solution is added into wells to react with enzyme and appears blue in color. The enzyme-substrate reaction is terminated by the addition of the stop solution, the color changes from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

  1.   Micro ELISA Plate   96T×1
  2.   Enzyme Conjugate   6mL×1
  3.   10×Concentrated wash solution   50mL×1
  4.   Color-substrate solution   11mL×1
  5.   Sample diluent   50mL×1
  6.   Stop solution   6mL×1
  7.   Positive control   0.8mL×1
  8.   Negative control   0.8mL×1
  9.   Serologecal Plate   1
  10.   Plate Sealer   2
  11.   Instruction   1

The kit is stored at 2~8℃.

      If you need the manual for more details, you can click here
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