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8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit

Cat.No.:E-EL-0041
Reactivity: Universal

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes 8-epi-PGF2α in samples. No significant cross-reactivity or interference between 8-epi-PGF2α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-epi-PGF2α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-epi-PGF2α were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.20	101.80	446.40	54.50	95.70	463.70
Standard deviation	2.90	4.50	15.60	3.50	5.00	25.00
CV (%)	5.66	4.42	3.49	6.42	5.22	5.39

Recovery

The recovery of 8-epi-PGF2α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-97	92
EDTA plasma(n=8)	92-108	100
Cell culture media(n=8)	93-107	100

Linearity

Samples were spiked with high concentrations of 8-epi-PGF2α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin
Research Area	Signal transduction, Cardiovascular

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

EPMA Journal (2021) IF: 6.543
Early gestational profiling of oxidative stress and angiogenic growth mediators as predictive, preventive and personalised (3P) medical approach to identify suboptimal health pregnant mothers likely to develop preeclampsia

DOI: 10.1007/s13167-021-00258-x

PMID: 34956424
Sample: serum
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2022) IF: 5.589
A green-inspired method to prepare non-split high-density lipoprotein (HDL) carrier with anti-dysfunctional activities superior to reconstituted HDL

DOI: 10.1016/j.ejpb.2022.12.005

Sample: Raw 264.7 cells
RESPIRATORY RESEARCH (2019) IF: 5.631
Amelioration of PM 2.5 -induced lung toxicity in rats by nutritional supplementation with fish oil and Vitamin E

DOI: 10.1186/s12931-019-1045-7

Sample: lung
Nutrients (2020) IF: 4.546
Total Dietary Antioxidant Intake Including Polyphenol Content: Is It Capable to Fight against Increased Oxidants within the Body of Ultra-Endurance Athletes?

DOI: 10.3390/nu12061877

PMID: 32586010
Sample: Plasma
BUILDING AND ENVIRONMENT (2020) IF: 4.971
Winter vacation, indoor air pollution and respiratory health among rural college students: A case study in Gansu Province, China

DOI: 10.1016/j.buildenv.2020.107481

Sample: Urine
Food & Nutrition Research (2016) IF: 3.226
Perinatal exposure to germinated brown rice and its gamma amino-butyric acid-rich extract prevents high fat diet-induced insulin resistance in first generation rat offspring

DOI: 10.3402/fnr.v60.30209

Sample: Serum
PLoS One (2022) IF: 3.24
Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia

DOI: 10.1371/journal.pone.0265717

PMID: 35312727
Sample: Serum
LIFE SCIENCES (2019) IF: 3.448
Thymoquinone and geraniol alleviate cisplatin-induced neurotoxicity in rats through downregulating the p38 MAPK/STAT-1 pathway and oxidative stress

DOI: 10.1016/j.lfs.2019.04.065

Sample: brain tissues
Journal of Diabetes Research (2015) IF: 2.164
Edible Bird’s Nest Prevents High Fat Diet-Induced Insulin Resistance in Rats

DOI: 10.1155/2015/760535

Sample: Serum
Animals (2020) IF: 2.323
Omega-3 Alpha-Linolenic Fatty Acid Affects the Level of Telomere Binding Protein TRF1 in Porcine Skeletal Muscle

DOI: 10.3390/ani10061090

Sample: Tissue homogenate
GERIATRIC NURSING (2022) IF: 2.525
Dietary diversity, diet quality, and oxidative stress in older adults

DOI: 10.1016/j.gerinurse.2022.09.013

PMID: 36219935
Sample: blood
Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
Antioxidant Activity of Sprouts Extracts Is Correlated with Their Anti-Obesity and Anti-Inflammatory Effects in High-Fat Diet-Fed Mice

DOI: 10.1155/2021/8367802

PMID: 33643424
Sample: Plasma
FREE RADICAL RESEARCH (2019) IF: 2.825
Suboptimal health pregnant women are associated with increased oxidative stress and unbalanced pro- and antiangiogenic growth mediators: a cross-sectional study in a Ghanaian population

DOI: 10.1080/10715762.2019.1685668

Sample: serum
LIFE SCIENCES (2017) IF: 2.936
Roselle is cardioprotective in diet-induced obesity rat model with myocardial infarction

DOI: 10.1016/j.lfs.2017.10.030

Sample: Tissue homogenate
Journal of General and Family Medicine (2023)
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial

DOI: 10.1002/jgf2.606

Sample: serum

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