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Cr(Creatinine) ELISA Kit

Cat.No.:E-EL-0058
Reactivity: Universal

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96T
48T
24T

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	1.25-80 μg/mL
Sensitivity	0.75 μg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes Cr in samples. No significant cross-reactivity or interference between Cr and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Cr concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cr. During the reaction, Cr in the sample or standard competes with a fixed amount of Cr on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cr. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cr in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Cr were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Cr were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (μg/mL)	3.90	10.70	34.80	4.20	11.10	31.60
Standard deviation	0.20	0.50	1.80	0.30	0.50	1.70
CV (%)	5.13	4.67	5.17	7.14	4.50	5.38

Recovery

The recovery of Cr spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-100	94
EDTA plasma(n=8)	92-108	100
Cell culture media(n=8)	94-107	99

Linearity

Samples were spiked with high concentrations of Cr and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms

2-Amino-1-methyl-5H-imidazol-4-one, 2-Amino-1-methylimidazol-4-ol

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
Empagliflozin reduces kidney fibrosis and improves kidney function by alternative macrophage activation in rats with 5/6-nephrectomy

DOI: 10.1016/j.biopha.2022.113947

PMID: 36411661
Sample: urine,plasma
Microbial Biotechnology (2022) IF: 6.575
Abelmoschus Manihot ameliorates the levels of circulating metabolites in diabetic nephropathy by modulating gut microbiota in non-obese diabetes mice

DOI: 10.1111/1751-7915.14200

Sample: urine
FASEB JOURNAL (2021) IF: 5.192
Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

DOI: 10.1096/fj.202101030R

PMID: 34516679
Sample: plasma
Metabolites (2022) IF: 5.581
Interaction between Plasma Metabolomics and Intestinal Microbiome in db/db Mouse, an Animal Model for Study of Type 2 Diabetes and Diabetic Kidney Disease

DOI: 10.3390/metabo12090775

PMID: 36144180
Sample: urine
Pharmaceuticals (2022) IF: 5.863
Fabrication and Evaluation of Quercetin Nanoemulsion: A Delivery System with Improved Bioavailability and Therapeutic Efficacy in Diabetes Mellitus

DOI: 10.3390/ph15010070

Sample: serum
Journal of Cell Communication and Signaling (2022) IF: 5.908
Prediction of cellular targets in diabetic kidney diseases with single-cell transcriptomic analysis of db/db mouse kidneys

DOI: 10.1007/s12079-022-00685-z

PMID: 35809207
Sample: urine
TOXICOLOGY (2022) IF: 4.221
Protective effects of Folic acid against reproductive, hematological, hepatic, and renal toxicity induced by Acetamiprid in male Albino rats

DOI: 10.1016/j.tox.2022.153115

PMID: 35124148
Sample: serum
European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
MiR-181-5p protects mice from sepsis via repressing HMGB1 in an experimental model

DOI: 10.26355/eurrev_202009_23063

PMID: 33015817
Sample: Serum
PLoS One (2021) IF: 3.24
Protective effect of a new generation of activated and purified bentonite in combination with yeast and phytogenic substances on mycotoxin challenge in pigs

DOI: 10.1371/journal.pone.0259132

PMID: 34705867
Sample: urine
NEFROLOGIA (2021) IF: 2.033
Can magnesium sulfate prophylaxis reduce colistin nephrotoxicity?

DOI: 10.1016/j.nefro.2020.11.020

PMID: 33892977
Sample: Plasma
Genetic Testing and Molecular Biomarkers (2020) IF: 1.378
Correlation Between Single Nucleotide Polymorphisms at the 3′-UTR of the NFKB1 Gene and Acute Kidney Injury in Sepsis

DOI: 10.1089/gtmb.2019.0222

Sample: Cell sample
TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635
Toxic effects of subchronic oral acetamiprid exposure in rats:

DOI: 10.1177/0748233719893203

Sample: plasma
BIOLOGICAL & PHARMACEUTICAL BULLETIN (2018) IF: 1.694
Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

DOI: 10.1248/bpb.b17-00930

PMID: 30068867
Sample: Plasma
Clinical and Experimental Nephrology (2020) IF: 1.77
Neointimal hyperplasia in the inferior vena cava of adenine-induced chronic kidney disease rats with aortocaval fistulas

DOI: 10.1007/s10157-020-01927-3

PMID: 32666345
Sample: Serum
INTERNATIONAL UROLOGY AND NEPHROLOGY (2020) IF: 1.843
Vinpocetine reduces cisplatin-induced acute kidney injury through inhibition of NF–κB pathway and activation of Nrf2/ARE pathway in rats

DOI: 10.1007/s11255-020-02485-z

Sample: Serum,Urine,Tissue homogenate
Molecular Medicine Reports (2019) IF: 1.851
Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney

DOI: 10.3892/mmr.2019.10576

Sample: serum
Acta Cirurgica Brasileira (2020) IF: 0.974
Effects of ATRA on diabetic rats with renal ischemia-reperfusion injury

DOI: 10.1590/s0102-865020200010000006

PMID: 32236320
Sample: Serum

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