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GnRH(Gonadotropin Releasing Hormone) ELISA Kit

Cat.No.:E-EL-0071
Reactivity: Universal

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes GnRH in samples. No significant cross-reactivity or interference between GnRH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GnRH. During the reaction, GnRH in the sample or standard competes with a fixed amount of GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GnRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GnRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GnRH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GnRH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	281.20	937.80	2978.30	281.90	856.30	3080.80
Standard deviation	14.60	48.80	131.00	18.00	41.10	114.00
CV (%)	5.19	5.20	4.40	6.39	4.80	3.70

Recovery

The recovery of GnRH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-111	101
EDTA plasma(n=8)	88-100	95
Cell culture media(n=8)	87-97	92

Linearity

Samples were spiked with high concentrations of GnRH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	GNRH1, GRH, HH12, LHRH, LNRH, Luliberin
Research Area	Developmental biology, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

Molecular Therapy-Nucleic Acids (2021) IF: 8.8859
The epigenetic role of HTR1A antagonist in facilitating GnRH expression for pubertal initiation control

DOI: 10.1016/j.omtn.2021.05.014

Sample: GT1-7 cells
SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
Effects of acute exposure to microcystins on hypothalamic-pituitary-adrenal (HPA), -gonad (HPG) and -thyroid (HPT) axes of female rats

DOI: 10.1016/j.scitotenv.2021.145196

PMID: 34030373
Sample: Serum
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291
Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice

DOI: 10.1016/j.ecoenv.2020.111748

PMID: 33396074
Sample: Serum
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291
In utero exposure to DBP stimulates release of GnRH by increasing the secretion of PGE2 in the astrocytes of the hypothalamus in the offspring mice

DOI: 10.1016/j.ecoenv.2020.110698

PMID: 32388187
Sample: Cell culture supernatant
International Journal of Nanomedicine (2022) IF: 6.4
Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway

DOI: 10.2147/IJN.S348673

PMID: 35401001
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway

DOI: 10.1016/j.biopha.2022.113061

PMID: 35658231
Sample: serum
Cells (2022) IF: 6.6
Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

DOI: 10.3390/cells11101690

PMID: 35626727
Sample: serum
ENVIRONMENTAL POLLUTION (2020) IF: 6.793
Perfluorooctanoic acid exposure impact a trade-off between self-maintenance and reproduction in lizards (Eremias argus) in a gender-dependent manner

DOI: 10.1016/j.envpol.2020.114341

Sample: Tissue homogenate
LIFE SCIENCES (2022) IF: 5.037
Studies on testicular ultrastructural and hormonal changes in type-2 diabetic rats treated with highly active antiretroviral therapy conjugated silver nanoparticles

DOI: 10.1016/j.lfs.2022.120498

PMID: 35341824
Sample: serum
LIFE SCIENCES (2021) IF: 5.037
Evaluation of maternal high-fat diet and Quercetin-3-O-rutinoside treatment on the reproductive profile of diet naïve male offspring

DOI: 10.1016/j.lfs.2021.119179

PMID: 33577849
Sample: Tissue homogenate
CHEMOSPHERE (2019) IF: 5.108
T-2 toxin impairs male fertility by disrupting hypothalamic-pituitary-testis axis and declining testicular function in mice

DOI: 10.1016/j.chemosphere.2019.06.145

PMID: 31519099
Sample: Serum
SCIENCE OF THE TOTAL ENVIRONMENT (2019) IF: 5.589
Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus

DOI: 10.1016/j.scitotenv.2019.02.419

PMID: 30833257
Sample: Plasma
ENVIRONMENTAL POLLUTION (2019) IF: 5.714
Enrichment of imidacloprid and its metabolites in lizards and its toxic effects on gonads

DOI: 10.1016/j.envpol.2019.113748

PMID: 31874432
Sample: Plasma
TOXICOLOGY (2021) IF: 4.221
Environmentally relevant perinatal exposure to DBP disturbs testicular development and puberty onset in male mice

DOI: 10.1016/j.tox.2021.152860

PMID: 34280466
Sample: Serum
TOXICOLOGY LETTERS (2021) IF: 4.374
Effects of prenatal chlorocholine chloride exposure on pubertal development and reproduction of male offspring in rats

DOI: 10.1016/j.toxlet.2021.08.005

PMID: 34411681
Sample: serum
BRAIN RESEARCH (2018) IF: 3.125
Intracerebroventricular injection of histamine induces the hypothalamic-pituitary-gonadal axis activation in male rats

DOI: 10.1016/j.brainres.2018.08.020

Sample: plasma
REPRODUCTIVE TOXICOLOGY (2021) IF: 3.143
Repression of HDAC5 by acetate restores hypothalamic-pituitary-ovarian function in type 2 diabetes mellitus

DOI: 10.1016/j.reprotox.2021.10.008

PMID: 34656705
Sample: plasma
Brain Sciences (2022) IF: 3.333
Exogenous Melatonin Regulates Puberty and the Hypothalamic GnRH-GnIH System in Female Mice

DOI: 10.3390/brainsci12111550

Sample: serum
TOXICOLOGY LETTERS (2019) IF: 3.499
Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats

DOI: 10.1016/j.toxlet.2019.10.024

PMID: 31689472
Sample: Serum
FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775
Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats

DOI: 10.1016/j.fct.2019.110897

PMID: 31654709
Sample: Serum
BMC Complementary and Alternative Medicine (2019) IF: 2.479
Cannabinoid-deficient Benin republic hemp ( Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

DOI: 10.1186/s12906-019-2541-5

Sample: plasma
Veterinary Research Forum (2021) IF: 1.054
Season influence on serum kisspeptin level and its association with hormonal levels and semen kinematics of buffalo bulls (Bubalus bubalis)

DOI: 10.30466/vrf.2020.122142.2873

Sample: serum
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL (2015) IF: 1.562
Brain thromboxane A2 via arachidonic acid cascade induces the hypothalamic–pituitary–gonadal axis activation in rats

DOI: 10.1016/j.autneu.2015.02.005

Sample: Plasma

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Reviews/Q&A

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Reviews
Q&A

A***********hSubmitted [ Jun 13 2023 ]

Asked: I would like a quote for this item, if you have a bovine GnRH kit, it is most appropriate for our assay GnRH(Gonadotropin Releasing Hormone) ELISA Kit Cat.No.:E-EL-0071 Also I would like to ask for a quote for GnRH-HRP conjugate detection reagent separately, Could you provide that separately please?

adminSubmitted [ Jun 13 2023 ]

Answered: Hello Ahmed, I will reply you via email later.

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DOI: 10.1016/j.scitotenv.2021.145196
PMID: 34030373	Sample: Serum

DOI: 10.1016/j.ecoenv.2020.111748
PMID: 33396074	Sample: Serum

DOI: 10.1016/j.ecoenv.2020.110698
PMID: 32388187	Sample: Cell culture supernatant

DOI: 10.2147/IJN.S348673
PMID: 35401001	Sample: serum

DOI: 10.1016/j.biopha.2022.113061
PMID: 35658231	Sample: serum

DOI: 10.3390/cells11101690
PMID: 35626727	Sample: serum

DOI: 10.1016/j.envpol.2020.114341
	Sample: Tissue homogenate

DOI: 10.1016/j.lfs.2022.120498
PMID: 35341824	Sample: serum

DOI: 10.1016/j.lfs.2021.119179
PMID: 33577849	Sample: Tissue homogenate

DOI: 10.1016/j.chemosphere.2019.06.145
PMID: 31519099	Sample: Serum

DOI: 10.1016/j.omtn.2021.05.014
	Sample: GT1-7 cells

DOI: 10.1016/j.scitotenv.2019.02.419
PMID: 30833257	Sample: Plasma

DOI: 10.1016/j.envpol.2019.113748
PMID: 31874432	Sample: Plasma

DOI: 10.1016/j.autneu.2015.02.005
	Sample: Plasma

DOI: 10.1016/j.tox.2021.152860
PMID: 34280466	Sample: Serum

DOI: 10.1016/j.toxlet.2021.08.005
PMID: 34411681	Sample: serum

DOI: 10.1016/j.reprotox.2021.10.008
PMID: 34656705	Sample: plasma

DOI: 10.1016/j.toxlet.2019.10.024
PMID: 31689472	Sample: Serum

DOI: 10.1016/j.fct.2019.110897
PMID: 31654709	Sample: Serum

GnRH(Gonadotropin Releasing Hormone) ELISA Kit

To Purchase E-EL-0071

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

Molecular Therapy-Nucleic Acids (2021) IF: 8.8859

SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291

International Journal of Nanomedicine (2022) IF: 6.4

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53

Cells (2022) IF: 6.6

ENVIRONMENTAL POLLUTION (2020) IF: 6.793

LIFE SCIENCES (2022) IF: 5.037

LIFE SCIENCES (2021) IF: 5.037

CHEMOSPHERE (2019) IF: 5.108

SCIENCE OF THE TOTAL ENVIRONMENT (2019) IF: 5.589

ENVIRONMENTAL POLLUTION (2019) IF: 5.714

TOXICOLOGY (2021) IF: 4.221

TOXICOLOGY LETTERS (2021) IF: 4.374

BRAIN RESEARCH (2018) IF: 3.125

REPRODUCTIVE TOXICOLOGY (2021) IF: 3.143

Brain Sciences (2022) IF: 3.333

TOXICOLOGY LETTERS (2019) IF: 3.499

FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775

BMC Complementary and Alternative Medicine (2019) IF: 2.479

Veterinary Research Forum (2021) IF: 1.054

AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL (2015) IF: 1.562

Reviews/Q&A

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