Your present position: Home > Product > ELISA kits > Human

Business hours:

Monday-Friday


After business hours or during company functions when telephones not answered, you may use our Email address or Livechat in the lower right corner to leave a message.


Tel: 1-240-252-7368

      1-240-252-7376

Fax:1-240-252-7376


Human ANG1 (Angiopoietin 1) ELISA Kit
Synonyms:ANG-1; ANGPT1; AGP1; AGPT

Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and then incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANG1 . You can calculate the concentration of Human ANG1 in the samples by comparing the OD of the samples to the standard curve.

Product ID E-EL-H0007
Assay type Sandwich ELISA
Format 96T
Assay time 4.5h
Reactivity Human
Detection Method Colormetric
Detection Range 62.500—4000.000pg/mL
Sensitivity 37.500pg/mL
Sample Volume Required Per Well 100μL
Sample Type serum, plasma and other biological fluids

Components

Item Specifications Storage
Micro ELISA Plate 8 wells ×12 strips 4℃/-20℃#
Reference Standard 2 vials 4℃/-20℃#
Reference Standard & Sample Diluent 1vial 20mL 4℃
Concentrated Biotinylated Detection Ab 1vial 120μL 4℃/-20℃#
Biotinylated Detection Ab Diluent 1vial 10mL 4℃
Concentrated HRP Conjugate 1vial 120μL 4℃(shading light)
HRP Conjugate Diluent 1vial 10mL 4℃
Concentrated Wash Buffer (25×) 1vial 30mL 4℃
Substrate Reagent 1vial 10mL 4℃(shading light)
 Stop Solution 1vial 10mL 4℃
Plate Sealer 5pieces
Manual 1 copy
Certificate of Analysis 1 copy
#: keep the kit at 4℃ if it’s used within 30 days, keep at -20℃ for longer storage.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human ANG1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human ANG1 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean
(pg/mL)
216.000 498.900 1629.900 226.500 533.800 1641.800
Standard deviation 14.300 20.000 60.300 15.400 27.200 50.900
C V (%) 6.600 4.000 3.700 6.800 5.100 3.100

Recovery

The recovery of Human ANG1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Range (%) Average Recovery (%)
Serum (n=5) 85-98 91.000
EDTA plasma (n=5) 91-104 96.000
Cell culture media (n=5) 93-110 100.000

Linearity

Samples were spiked with high concentrations of Human ANG1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

  Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 87-101 94-108 98-111
Average (%) 92.000 100.000 105.000
1:4 Range (%) 96-108 81-93 91-104
Average (%) 101.000 87.000 98.000
1:8 Range (%) 95-113 85-95 99-112
Average (%) 103.000 90.000 105.000
1:16 Range (%) 98-114 82-92 99-111
Average (%) 106.000 87.000 105.000

Specificity

This kit recognizes recombinant and natural Human ANG1 . No significant cross-reactivity or interference was observed.

Procedure

  1. Add 100μL standard or sample to each well. Incubate 90 mintues at 37℃.
  2. Add 100μL Biotinylated Detection Ab. Incubate 1 hour at 37℃.
  3. Aspirate and wash 3 times.
  4. Add 100μL HRP Conjugate. Incubate 30 minutes at 37℃.
  5. Aspirate and wash 5 times.
  6. Add 90μL Substrate Reagent. Incubate 15 minutes at 37℃.
  7. Add 50μL Stop Solution. Read it at 450nm immediately.
  8. Calculation of results.

Storage

All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20℃ (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination.

If you need the manual for more details, you can click here! 

Region Name Email
North America John
Alice
john@elabscience.com
alice@elabscience.com
South America Gwyneth Gwyneth@elabscience.com
Asia Britney britney@elabscience.com
Japan & Taiwan Yumi yumi.elabscience@gmail.com
South Asia & Australia Hanny hanny@elabscience.com
Eastern & Northern Europe Cathy cathy@elabscience.com
Southern Europe & Africa Heidi heidi@elabscience.com
Western & Middle Europe Perry Perry.elabscience@gmail.com
Phone:
240-252-7368(USA)    240-252-7376(USA)