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This kit uses Indirect-Competitive-ELISA as the method. It is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The microplate provided in this kit has been pre-coated with DES. During the reaction, DES in the samples or standard competes with DES on the solid phase supporter for sites of DES antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development, There is a negative correlation between the OD value of samples and the concentration of DES. You can calculate the concentration of DES in the samples by comparing the OD of the samples to the standard curve.
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