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This kit uses Indirect-Competitive-ELISA as the method.It is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The microplate provided in this kit has been pre-coated with DON. During the reaction, DON in the samples or standard competes with DON on the solid phase supporter for sites of DON antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development, There is a negative correlation between the OD value of samples and the concentration of DON. You can calculate the concentration of DON in the samples by comparing the OD of the samples to the standard curve.
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