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This kit uses Indirect-Competitive-ELISA as the method.It is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The microplate provided in this kit has been pre-coated with AFM1. During the reaction, AFM1 in the samples or standard competes with AFM1 on the solid phase supporter for sites of AFM1 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of AFM1. You can calculate the concentration of AFM1 in the samples by comparing the OD of the samples to the standard curve.
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