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Cat.No.:E-EL-H0109
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 12 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 12 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TNF-α were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 24.10 | 79.50 | 183.00 | 22.70 | 86.50 | 177.40 |
Standard deviation | 1.50 | 3.80 | 9.70 | 1.20 | 4.00 | 7.60 |
CV (%) | 6.22 | 4.78 | 5.30 | 5.29 | 4.62 | 4.28 |
The recovery of Human TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-100 | 92 |
EDTA plasma (n=8) | 94-106 | 99 |
Cell culture media (n=8) | 93-107 | 99 |
Samples were spiked with high concentrations of Human TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P01375
|
Synonyms | DIF, TNF-alpha, TNFA, TNFSF2 |
Research Area | Cancer, Cardiovascular, Metabolism, Immunology, Signal transduction |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
DOI: 10.1155/2021/6695679 |
|
PMID: 33628853 |
Sample: Cell Culture Supernatant(Hmecs) |
Sample: Hela Cell |
Sample: Nucleus Pulposus (Np) Cell |
PMID: 36091773 |
Sample: U87 Cell |
Sample: Thp-1 Cells |
Sample: Hmc3 Cells |
Sample: Beas-2B Cell |
DOI: 10.1155/2022/6181448 |
|
PMID: 36248627 |
Sample: Pc3 Cell,Hsf Cell |
DOI: 10.1002/ptr.7113 |
|
PMID: 33899292 |
Sample: Serum |
PMID: 30448228 |
Sample: Cell Culture Supernatant |
DOI: 10.1111/liv.14375 |
|
Sample: Plasma |
DOI: 10.2147/JBM.S305566 |
|
Sample: Os-Scs Cells |
Sample: Plasma,Serum |
Sample: Hacat Cells |
Sample: Plasma |
Sample: Serum |
PMID: 32276657 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Serum |
PMID: 31531603 |
Sample: Cell Culture Supernatant |
PMID: 30562142 |
Sample: Plasma |
DOI: 10.1159/000505776 |
|
Sample: Serum |
Sample: Cell Culture Supernatant |
Sample: Immunosensor Construction |
Sample: Serum |
DOI: 10.1111/ans.15767 |
|
PMID: 32115876 |
Sample: Serum,Urine |
Sample: Cell Sample |
PMID: 31938089 |
Sample: Beas2B Cells |
Sample: Cell Culture Supernatant |
Sample: Peripheral Blood |
Sample: Cell Culture Supernatant |
Sample: Serum |
PMID: 30988754 |
Sample: Plasma |
DOI: 10.1093/jbcr/irz029 |
|
PMID: 30926991 |
Sample: Serum |
DOI: 10.1159/000511711 |
|
Sample: Serum |
PMID: 33080678 |
Sample: Cell Culture Supernatant |
Sample: Cell Culture Supernatant |
PMID: 34139308 |
Sample: Hnecs Cells |
Sample: Serum |
Sample: Cell Culture Supernatant |
Sample: Serum |
DOI: 10.1155/2020/5834502 |
|
PMID: 32714409 |
Sample: Cell Culture Supernatant |
Sample: Cell Culture Supernatant |
Sample: Plasma |
Sample: Serum |
DOI: 10.12659/MSM.910740 |
|
Sample: Serum |
DOI: 10.12659/MSM.908592 |
|
Sample: Serum |
PMID: 32556004 |
Sample: Serum |
DOI: 10.12659/MSM.921957 |
|
PMID: 32463805 |
Sample: Cell Culture Supernatant |
Sample: Cell Culture Supernatant |
PMID: 32805637 |
Sample: Cell Culture Supernatant |
DOI: 10.1155/2019/5961742 |
|
PMID: 30941196 |
Sample: Cell Culture Supernatant |
PMID: 34677101 |
Sample: Mesenchymal Stem Cells (Mscs) |
PMID: 32036960 |
Sample: Plasma |
Sample: Serum |
Sample: Plasma |
PMID: 33300058 |
Sample: Cell Culture Supernatant |
PMID: 32924613 |
Sample: Serum |
DOI: 10.2147/IJGM.S372038 |
|
PMID: 35898299 |
Sample: Plasma |
PMID: 32840731 |
Sample: Tissue Homogenate |
DOI: 10.1155/2019/3619357 |
|
Sample: Salivary |
DOI: 10.1111/apm.12925 |
|
Sample: Cell Culture Supernatant |
Sample: Serum |
Sample: Serum |
DOI: 10.1155/2020/8748934 |
|
Sample: Serum |
PMID: 34521466 |
Sample: Blood |
PMID: 33541789 |
Sample: Cell Culture Supernatant |
PMID: 35246021 |
Sample: Plasma |
PMID: 34517822 |
Sample: Vec |
DOI: 10.1002/jmv.27294 |
|
PMID: 34427934 |
Sample: Serum |
DOI: 10.7717/peerj.7079 |
|
Sample: Urinary,Serum |
PMID: 35331286 |
Sample: Chondrocytes |
Sample: Cell Culture Supernatant |
Sample: Blood |
Sample: Cell Lysate |
PMID: 31473895 |
Sample: Cell Lysate |
PMID: 35240291 |
Sample: Serum |
DOI: 10.21037/tp-21-195 |
|
Sample: Serum |
DOI: 10.1159/000513330 |
|
Sample: Hcnepcs |
DOI: 10.1002/ppul.25097 |
|
PMID: 32986303 |
Sample: Plasma |
DOI: 10.1042/BSR20181202 |
|
Sample: Serum |
PMID: 32669002 |
Sample: Serum |
PMID: 31493501 |
Sample: Cell Culture Supernatant |
DOI: 10.1155/2022/5469236 |
|
PMID: 35873363 |
Sample: Ges-1 Cell |
DOI: 10.21037/apm-21-2168 |
|
Sample: Blood |
DOI: 10.21037/apm-21-1056 |
|
Sample: Serum |
Sample: Sh-Sy5Y Cells |
Sample: Serum |
DOI: 10.1155/2021/9958808 |
|
Sample: Thp-1 Cells |
Sample: Human Umbilical Vein Endothelial Cells (Huvecs) |
DOI: 10.1155/2022/9092311 |
|
Sample: Human Nasal Epithelial Cell (Hnecs) |
DOI: 10.1155/2022/2604114 |
|
PMID: 36034961 |
Sample: Serum |
DOI: 10.3892/or.2016.5216 |
|
Sample: Macrophage |
PMID: 36252429 |
Sample: Sk-N-Sh Cells |
DOI: 10.3390/app112110395 |
|
Sample: Dental Pulp Lysates |
DOI: 10.3390/app12031554 |
|
Sample: Dental Pulp Tissue |
DOI: 10.1155/2022/2961187 |
|
PMID: 35281537 |
Sample: Hk-2 Cell |
PMID: 31668807 |
Sample: Cell Culture Supernatant |
DOI: 10.1111/sji.12868 |
|
PMID: 32052490 |
Sample: Serum |
Sample: Plasma |
PMID: 35016790 |
Sample: Plasma |
Sample: Cell Culture Supernatant,Serum |
Sample: Serum |
DOI: 10.3967/bes2022.089 |
|
PMID: 35945185 |
Sample: Thp-1 Cell |
DOI: 10.1159/000518172 |
|
PMID: 34518457 |
Sample: Hrgecs |
DOI: 10.1111/dth.14733 |
|
PMID: 33389789 |
Sample: Serum |
Sample: Serum |
PMID: 35903285 |
Sample: Kidney Tissue |
Sample: Human Retinal Endothelial Cell |
PMID: 33955515 |
Sample: Cell Culture Supernatant |
Sample: Serum |
DOI: 10.7717/peerj.11694 |
|
PMID: 34414022 |
Sample: Plasma |
DOI: 10.7717/peerj.12934 |
|
PMID: 35186501 |
Sample: Serum |
PMID: 33341068 |
Sample: Cell Culture Supernatant |
Sample: Serum |
PMID: 32335809 |
Sample: Serum |
Sample: Cell Culture Supernatant |
Sample: Plasma |
Sample: Serum |
PMID: 30416660 |
Sample: Plasma |
Sample: Serum |
PMID: 30634164 |
Sample: Cell Lysate |
PMID: 34369900 |
Sample: Huvec Cells |
Sample: Serum |
PMID: 30476824 |
Sample: Cell Culture Supernatant |
Sample: Hepg2 Cells |
Sample: Hek293T Cells |
DOI: 10.3390/ijms18081614 |
|
Sample: Cell Culture Supernatant |
DOI: 10.1155/2017/3605350 |
|
Sample: Serum |
PMID: 30550887 |
Sample: Cell Culture Supernatant |
PMID: 31150725 |
Sample: Serum |
Sample: Plasma |
PMID: 35687545 |
Sample: Serum |
DOI: 10.1155/2022/6006981 |
|
PMID: 36330453 |
Sample: Htr-8/Svneo Cells,Jeg-3 Cells |
DOI: 10.1002/jgm.3236 |
|
PMID: 32468600 |
Sample: Serum |
PMID: 35236245 |
Sample: Huvecs |
Sample: Beas-2B Cell |
PMID: 35006038 |
Sample: Hacat Cell |
PMID: 35510503 |
Sample: Human Retinal Microvascular Endothelial Cell (Hrec |
PMID: 34898371 |
Sample: Arpe-19 Cell |
Sample: Thp-1 Cells |
Sample: Mcf-7 Cell,Amj-13 Cell |
PMID: 35933083 |
Sample: Serum |
DOI: 10.2147/OTT.S265859 |
|
Sample: Cell Culture Supernatant |
Sample: Cell Culture Supernatant |
PMID: 35980503 |
Sample: Thp-1 Cell,H1299 Cell |
Sample: Serum |
Sample: Serum,Cell Sample |
PMID: 34299679 |
Sample: Saliva |
PMID: 33885954 |
Sample: Cell Culture Supernatant |
DOI: 10.5937/jomb0-33096 |
|
Sample: Hk-2 Cell |
PMID: 31783135 |
Sample: Cell Culture Supernatant |
Sample: Sputum |
DOI: 10.1002/jcb.28724 |
|
Sample: Rheumatoid Arthritis (Ra) Fibroblast-Like Synovioc |
PMID: 34276038 |
Sample: Serum |
PMID: 35279426 |
Sample: Cerebrospinal Fluid (Csf) |
PMID: 33705895 |
Sample: Cell Culture Supernatant |
Sample: Serum |
PMID: 34999090 |
Sample: Lymphocytes,Macrophages |
Sample: Sh-Sy5Y Cell |
PMID: 36121373 |
Sample: Serum |
PMID: 36179036 |
Sample: Fecal |
Sample: Cell Culture Supernatant |
PMID: 32497675 |
Sample: Serum,Cell Culture Supernatant |
PMID: 30844656 |
Sample: Wound Drainage Fluid |
PMID: 32467068 |
Sample: Serum |
Sample: A549 Cell |
DOI: 10.3390/life11040272 |
|
Sample: Sw1353 Cell |
PMID: 31440860 |
Sample: Serum |
Sample: Serum |
Sample: Thp-1 Cell |
DOI: 10.21037/atm-21-4479 |
|
PMID: 34790733 |
Sample: Serum,Beas-2B Cell |
DOI: 10.21037/atm-20-7315 |
|
PMID: 33987242 |
Sample: Serum |
PMID: 33217691 |
Sample: Cell Culture Supernatant |
DOI: 10.1111/nmo.14512 |
|
Sample: Serum |
Sample: Serum |
PMID: 33221374 |
Sample: Cell Culture Supernatant |
Sample: Cell Culture Supernatant |
PMID: 32669704 |
Sample: Cell Culture Supernatant |
DOI: 10.1111/ijpo.12872 |
|
PMID: 34881510 |
Sample: Serum |
PMID: 35370905 |
Sample: Serum |
PMID: 32950783 |
Sample: Cell Culture Supernatant |
PMID: 35461393 |
Sample: Arpe-19 Cell |
PMID: 34664683 |
Sample: Peripheral Blood |
DOI: 10.1002/tox.23464 |
|
PMID: 35006638 |
Sample: A549 Cell |
PMID: 33469792 |
Sample: Plasma |
PMID: 34216342 |
Sample: Serum |
PMID: 33384597 |
Sample: Plasma |
DOI: 10.2147/IJN.S134897 |
|
Sample: Cell Culture Supernatant,Serum |
PMID: 30826466 |
Sample: Serum |
Sample: Serum,Hk-2 Cell |
DOI: 10.1002/ddr.21864 |
|
PMID: 34368980 |
Sample: Serum |
DOI: 10.5551/jat.63327 |
|
PMID: 36171087 |
Sample: Asmcs |
PMID: 34237073 |
Sample: Pleural Fluid |
DOI: 10.1111/jre.12995 |
|
PMID: 35468224 |
Sample: Gingival Crevicular Fluid (Gcf) |
Sample: Huvecs Cells |
Sample: Cell Sample |
PMID: 35218463 |
Sample: Blood |
Sample: Cell Culture Supernatant |
PMID: 32145584 |
Sample: Cell Culture Supernatant |
DOI: 10.3390/ijms21093228 |
|
Sample: Serum |
PMID: 32585358 |
Sample: Cell Culture Supernatant |
DOI: 10.1155/2017/8519169 |
|
Sample: Cell Culture Supernatant |
PMID: 36078325 |
Sample: Pc12 Cell |
DOI: 10.1167/iovs.62.9.12 |
|
PMID: 34236384 |
Sample: Hcecs Cells |
DOI: 10.1155/2021/1125199 |
|
PMID: 34595242 |
Sample: Serum |
Sample: Cell Culture Supernatant |
Sample: Cell Culture Medium |
PMID: 32303555 |
Sample: Cell Culture Supernatant |
Sample: Pbmcs Cells |
PMID: 34982483 |
Sample: Thp-1 Cell |
PMID: 33404078 |
Sample: Serum |
DOI: 10.1155/2021/6629455 |
|
PMID: 33968146 |
Sample: Serum |
Sample: Serum |
DOI: 10.1111/cpr.12673 |
|
Sample: Caco2 Cells,Ht‐29 Cell |
Sample: Blood |
DOI: 10.1042/CS20200047 |
|
PMID: 32219341 |
Sample: Cell Culture Supernatant |
DOI: 10.1039/C9BM01000A |
|
Sample: Thp-1 Cells |
Sample: Cell Culture Supernatant |
Sample: Thp-1 Cells |
Sample: Cell Culture Supernatant |
DOI: 10.1111/jcmm.17062 |
|
PMID: 34786826 |
Sample: Serum |
DOI: 10.1111/jcmm.16959 |
|
PMID: 34601814 |
Sample: Myocardial Tissue,Blood |
DOI: 10.1039/D1FO03896F |
|
Sample: Serum |
PMID: 35688467 |
Sample: Human Monocyte-Derived Dendritic Cells (Dcs) |
Sample: Serum |
DOI: 10.1002/jcp.29713 |
|
PMID: 32385929 |
Sample: Cell Culture Supernatant |
DOI: 10.1038/srep15132 |
|
Sample: Serum |
Sample: Serum |
Sample: Plasma,Cerebrospinal Fluid |
PMID: 35551614 |
Sample: A549 Cell |
Sample: Caco-2 Cell |
Sample: Hacat Cells |
PMID: 34720537 |
Sample: Serum |
Sample: Cell Culture Supernatant |
Sample: L02 Cell |
PMID: 35144130 |
Sample: A549 Cell |
PMID: 34829612 |
Sample: Saliva |
DOI: 10.1039/D2FO00477A |
|
Sample: Serum |
PMID: 33895109 |
Sample: Plasma |
PMID: 36145700 |
Sample: Hacat Cell |
PMID: 34311530 |
Sample: Serum |
DOI: 10.1155/2021/5584447 |
|
PMID: 34239691 |
Sample: Np Cells |
PMID: 34602194 |
Sample: Serum |
Sample: Serum |
DOI: 10.3390/nu14245240 |
|
Sample: Macrophage |
DOI: 10.3390/nu15030661 |
|
Sample: Serum |
DOI: 10.3390/nu15010194 |
|
Sample: Serum |
DOI: 10.3390/nu14142895 |
|
PMID: 35889856 |
Sample: Serum |
Sample: Serum |
DOI: 10.2147/JIR.S317182 |
|
Sample: U937 Cell |
DOI: 10.1002/JPER.21-0137 |
|
PMID: 33913157 |
Sample: Gingival Crevicular Fluid |
Sample: Hepg2 Cell |
Sample: Plasma,Thp-1 Cell |
PMID: 34875345 |
Sample: Serum |
DOI: 10.1155/2022/3576892 |
|
PMID: 35855864 |
Sample: Serum |
DOI: 10.1155/2022/4631983 |
|
Sample: Plasma |
Sample: Serum |
Sample: Thp-1 Monocytes |
PMID: 34721416 |
Sample: Serum |
Sample: Hmc3 Cells |
Sample: Cell Culture Supernatant |
DOI: 10.1039/D1NR00132A |
|
PMID: 33710220 |
Sample: Serum,Cell Culture Supernatant(Dendritic Cells,4T1 |
Sample: Hs68 Cell |
Sample: Thp-1 Cells |
PMID: 33986259 |
Sample: Cell Culture Supernatant |
PMID: 34999169 |
Sample: Serum,Thp-1 Cell,Human Circulating Cd14+ Monocytes |
Sample: Cardiac Microvascular Endothelial Cell (Cmecs),Car |
PMID: 33981482 |
Sample: Plasma |
PMID: 33738142 |
Sample: Cell Culture Supernatant(Htr-8/Svneo Cells,Jeg-3 C |
PMID: 35768823 |
Sample: Cerebrospinal Fluid (Csf),Peripheral Monocytes |
PMID: 36155221 |
Sample: Hmc3 Cell |
Sample: Perianal Fistula |
PMID: 35248068 |
Sample: Serum |
PMID: 33168024 |
Sample: Cell Culture Supernatant |
DOI: 10.7150/thno.58321 |
|
PMID: 33995679 |
Sample: Tissue Homogenate |
Sample: Cell Culture Supernatant |
DOI: 10.1002/hep.31056 |
|
PMID: 31808577 |
Sample: Serum |
PMID: 33015716 |
Sample: Cell Culture Medium |
Verified Customer
P******* Submitted [ Jan 17 2023 ]
a***lSubmitted [ Jul 17 2024 ]
Asked: Do the protocol needs high speed centrifugation? What is the rpm to spin?
Reply
adminSubmitted [ Jul 17 2024 ]
Answered: Lyophilized Standard requires high-speed centrifugation, Centrifuge the standard at 10,000×g for 1 min.
a***lSubmitted [ Jul 17 2024 ]
Asked: Do the protocol needs high speed centrifugation? What is the rpm to spin?
Reply
adminSubmitted [ Jul 17 2024 ]
Answered: Lyophilized Standard requires high-speed centrifugation, Centrifuge the standard at 10,000×g for 1 min.
Z******rSubmitted [ Jun 30 2024 ]
Asked: For how many subjects is one kit enough?
Reply
adminSubmitted [ Jun 30 2024 ]
Answered: Hello Zvonimir, Thanks for your question. I will send email to you about the answer details. Please check your email later. Have a nice day! Best regards, Sophie International Sales Manager Email: sophie@elabscience.com www.linkedin.com/in/elabscience Elabscience Bionovation Inc. www.elabscience.com US Tel: 240-252-7368 China Tel: 86-27-87385095 Fax: +1 832 243 6017 Mobile: +86 136 5989 1476 (WhatsApp, Wechat, Skype) l 14780 Memorial Drive, Suite 105, Houston, Texas 77079
S***aSubmitted [ Feb 14 2023 ]
Asked: \"For the kit E-EL-H0109, we have validated healthy human serum samples with the kit, the reference values of healthy human serum samples are below 30 pg/mL with individual differences.\" Is there any references that I can look up to?
Reply
adminSubmitted [ Feb 14 2023 ]
Answered: Hi,Sofia. I have replied you by email. Pls check the attachment of your mailbox.
A***********nSubmitted [ Aug 08 2022 ]
Asked: Is there a reference range for normal individuals for this KIT?
Reply
adminSubmitted [ Aug 08 2022 ]
Answered: For the kit E-EL-H0109, we have validated healthy human serum samples with the kit, the reference values of healthy human serum samples are below 30 pg/mL with individual differences.
S*********nSubmitted [ Aug 05 2021 ]
Asked: My kit got expired in April 2020. Shall we perform the experiment with the kit?
Reply
adminSubmitted [ Aug 05 2021 ]
Answered: Thanks for your message. The expired kit can be used to perform experiment anymore. By the way, where did you ordered the kit from?