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FAQs for ELISA
You can visit our website to see if there is a distributor in your area. If there is no one, you can contact us directly.
We would prepare for your order the moment we receive your pre-payment, and it will take 2-3 days before our delivering.
Yes, but our sample was provided on the condition that you are ready to place a big order and you want to check whether our products are qualified and suitable to your experiments. Besides, you have to bear the express cost for the free samples.
We only receive bank transfer at the moment.
Normally six months, but we receive special order.
Yes, we have an email catalogue, please contact us for details.
We usually use Fedex, UPS, DHL, TNT, aramex, EMS to deliver our goods. And you could be free to choose any express company which you have an account.
No, not unless the product specific instruction manual specifically gives you this option. A microtiter plate reader capable of detecting at the appropriate wavelength is needed to evaluate your data for most enzyme immunoassay and ELISA kits. The sample volume is too small to be reliably evaluated in a spectrophotometer.
Sample Matrices and Species
If your samples contain analyte levels beyond the dynamic range of the assay, you may dilute your samples and run directly in the kit. If your sample levels are below the limit of quantitation of the assay, extraction and concentration is necessary.
It is normal to see a small variation in OD values between experiments. OD values can be affected by small fluctuations in temperature. To assure that this is not a problem, do not leave the plate on a cold lab bench or under a vent for room temperature incubations. The relative level of signal can be improved by running the substrate incubation at 25 - 37°C.
For the most accurate results we recommend that 4 parameter logistic (4-PL) curve fitting software be used. The object of 4-PL is to find the line equation that best fits the data within a certain set of limitations. Even in immunometric assays, the dose response curve is very rarely a straight line. By choosing a better curve fit for your data, you will ultimately have more accurate returned sample values. If this software is unavailable the user will have to plot their data and calculate by hand. Most microplate readers come with software.