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Tel: 1-240-252-7368

      1-240-252-7376

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FAQs for ELISA


Author:Elabscience
views:1084


Order


1 How can I buy your products in my area?
 
You can visit our website to see if there is a distributor in your area. If there is no one, you can contact us directly.


2 How long will it take to receive my order?
 
We would prepare for your order the moment we receive your pre-payment, and it will take 2-3 days before our delivering.


3 Could I get a free sample?
 
Yes, but our sample was provided on the condition that you are ready to place a big order and you want to check whether our products are qualified and suitable to your experiments. Besides, you have to bear the express cost for the free samples.


4 What payment methods do we accept?
 
We only receive bank transfer at the moment.


5 How long do your products last?
 
Normally six months, but we receive special order.


6 Do you have a catalogue?
 
Yes, we have an email catalogue, please contact us for details.


7 Where is my package?
 
We usually use Fedex, UPS, DHL, TNT, aramex, EMS to deliver our goods. And you could be free to choose any express company which you have an account.


We would keep you informed of the track info and at the same time, we will offer the Shipment Number through email once we deliver the goods, you could track the orders on the website of the express company.


Fedex:

http://www.fedex.com

UPS:

http://www.ups.com

DHL:

http://www.cn.dhl.com/zh.html

TNT:

http://lit2.tnt.com.cn/tracker/trackandtraceInit.do

Aramex:

http://www.aramex.com

EMS:

http://www.ems.com.cn

Instrumentation

1 Do I have to use a wavelength correction?
Reading at dual wavelengths corrects for the optical density contributed by the plastic the plate wells are made of as well as lamp and instrument fluctuations. Our plates are chosen for their optical quality, therefore this correction is actually very small. If the dual wavelength feature is not available, data read at the recommended detection wavelength should be largely unaffected.  

2 Can I use a spectrophotometer to read my assay?
No, not unless the product specific instruction manual specifically gives you this option. A microtiter plate reader capable of detecting at the appropriate wavelength is needed to evaluate your data for most enzyme immunoassay and ELISA kits. The sample volume is too small to be reliably evaluated in a spectrophotometer.

 

Sample Matrices and Species  

1 What species does your kit work with?
Kits where the molecule is conserved among all species (e.g. eicosanoids, steroids) are species independent, and may be used with any species. Kits that list a specific species in the name (e.g. human) are specific for that species. If the species is not specified in the manual, the product is likely species independent. The information may also be found on the product specific page on our website.

2 How do I know if I need to dilute or extract my samples?
If your samples contain analyte levels
beyond the dynamic range of the assay, you may dilute your samples and run directly in the kit. If your sample levels are below the limit of quantitation of the assay, extraction and concentration is necessary.  

Data Analysis

1 Can you explain why I am seeing inconsistent standard curves run in culture media?
Often times, the serum supplemented into media can be problematic. In order to minimize this problem it is very important that the non-conditioned medium is at room temperature and is well-mixed before using to prepare your standard dilutions. Serum is more dense than water and tends to settle to the bottom of the vessel if not well mixed.

2 Why are my ODs different than those shown in the instruction manual?
It is normal to see a small variation in OD values between experiments. OD values can be affected by small fluctuations in temperature. To assure that this is not a problem, do not leave the plate on a cold lab bench or under a vent for room temperature incubations. The relative level of signal can be improved by running the substrate incubation at 25 - 37°C.

3 What is the best way to analyze my data? What is 4-PL curve-fitting?
For the most accurate results we recommend that 4 parameter logistic (4-PL) curve fitting software be used. The object of 4-PL is to find the line equation that best fits the data within a certain set of limitations. Even in immunometric assays, the dose response curve is very rarely a straight line. By choosing a better curve fit for your data, you will ultimately have more accurate returned sample values. If this software is unavailable the user will have to plot their data and calculate by hand. Most microplate readers come with software.