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Some tips for ELISA failure
ELISA failure can be attributed to many factors. Many unexpected results can be avoided if the manual is read and fully understood before starting the experiment. On identifying assay failure, you can follow tips below.
1. Check the expiration dates of the kit, ensure that all the reagents have been stored as indicated in the manual.
2. Check for signs of instability or deterioration in reagent solutions, such as precipitation or discoloration and so on.
3. Substrate reagent should be colorless.
4. Use clean plastic disposable pipettes, tips, and containers for reagent preparation and storage. Avoid cross-contamination of reagents by changing pipette tips between addition of each standard, sample and reagent.
5. Ensure that specified incubation times and temperatures have been adhered to.
6. Ensure that no substitution of kit reagents has occurred.
7. It is recommended that samples and standards be run in duplicate to improve accuracy.