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Poor Standard Curve in ELISA experiment
|Improper dilution of standard|
Use diluent in the kit as blank value.
Ensure accurate dilution of highest standard
Ensure accurate completion of 2-fold dilution series
Ensure that wash apparatus is working correctly
|Inadequate aspiration of wells||Wells should appear dry after aspiration|
|Unequal volumes added to wells|
Check pipette function;
Recalibrate the pipetter if necessary
|Pipetting error||Set duplicate in each assay|