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Poor Standard Curve in ELISA experiment


Author:Elabscience
views:588

Possible causes
Solutions
Improper dilution of standard
Use diluent in the kit as blank value.
Ensure accurate dilution of highest standard
Ensure accurate completion of 2-fold dilution series
Incomplete washing
Ensure that wash apparatus is working correctly
Inadequate aspiration of wellsWells should appear dry after aspiration
Unequal volumes added to wells
Check pipette function;
Recalibrate the pipetter if necessary
Pipetting errorSet duplicate in each assay



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