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Poor reproducibility in ELISA experiment


Author:Elabscience
views:557

Possible causes
Solutions
Insufficient washing
Ensure sufficient washing
If using an automatic plate washer, check that all ports are clean and free of obstructions
Variations in incubation temperature
Adhere to recommended incubation temperature
Avoid puting plates in vary environmental conditions
Plate sealer reused, resulting in presence of residual HRPUse fresh plate sealer for each step
Improper calculation of standard curve dilutionsCheck calculations, make new standard curve
Buffers contaminatedMake fresh buffers







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