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Pipetting Tips for researchers
The key to repeated accuracy in pipetting is a consistent technique. Consistent speed and smoothness is necessary while pipetting. Avoid sudden motions when drawing or dispensing liquid.
1. Check to be sure that the pipette tip is fitted properly on the end of the pipette and seated with adequate pressure
2. Change pipette tips between each sample or reagent addition.
3. Do not pipette a lower volume than the manufacturer’s suggestion. This will decrease accuracy and reproducibility.
4. Some liquids will have a tendency to cling to the inside or outside surfaces of the pipette tips. To avoid variations in the volumes drawn or dispensed caused by this surface tension, pre-rinse the pipette tip with the sample or reagent. This will improve accuracy.
5. If the reagent to be pipetted is viscous, wait a moment after drawing the liquid to allow the volume to reach equilibrium before removing the pipette tip from the reservoir.
6. After drawing the liquid into the tip, remove the tip from the solution and wipe the outside of the tip with a lint-free tissue.
7. If an air bubble appears in the tip while drawing the liquid, dispense the liquid back into the container and re-pipette the liquid at a slower rate. If the air bubble appears a second time, discard the pipette tip and replace with a new tip.
8. If foaming occurs in the tip while drawing up the liquid, it may be necessary to hold the pipette at a slight angle and draw the liquid slower.
9. Use separate reservoirs for each reagent.