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Sample collection and storage


Author:Elabscience
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Samples should be clear and transparent and be centrifuged to remove suspended solids.

Serum: Allow samples to clot for 2 hours at room temperature or overnight at 4℃ before centrifugation for 15 minutes at 1000×g. Collect the supernatant and carry out the assay immediately.Blood collection tubes should be disposable,non-pyrogenic, and non-endotoxin.

Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 - 8℃ within 30 minutes of collection. Collect the supernatant and carry out the assay immediately. Hemolysis samples are not suitable for ELISA assay!

Cell culture supernate: Centrifuge supernate for 20 minutes to remove insoluble impurity and cell debris at 1000×g at 2 - 8°C. Collect the clear supernate and carry out the assay immediately.

Tissue homogenates:You’d better get detailed references from other literatures before assay aiming at different tissue types. For general information, hemolysis blood may affect the result, so you should mince the tissues to small pieces and rinse them in ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then homogenized in PBS (the volume depends on the weight of the tissue) with a glass homogenizer on ice. To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifugated for 5 minutes at 5000×g to get the supernate.

Other biological fluids: Centrifuge samples for 20 minutes at 1000×g at 2 - 8℃. Collect the supernatant and carry out the assay immediately.

Note:

1. Samples should be used within 7 days when stored at 2-8℃, otherwise samples must be divided and stored at -20℃ (≤1month) or -80℃ (≤6months) to avoid the loss of bioactivity and contamination. Avoid repeated freeze-thaw cycles.

2. Please take the samples to room temperature (18-25 ℃ )without extra heating before performing the assay.

3. Please predict the concentration before assaying. If the sample concentration is not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.