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Cat.No.:E-BC-K094-S
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Protocols for metabolism assays
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Synonyms | ACP |
Sample type | Serum,plasma,urine,tissue,cells |
Sensitivity | 0.27 U/100 mL |
Detection range | 0.27-40 U/100 mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 70 min |
Precision | Average inter-assay CV: 7.100%Average intra-assay CV: 2.800% |
Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.27-40 U/100 mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human milk |
1 |
Human saliva |
1 |
Human urine |
1 |
Human serum |
1 |
HepG2 cells homogenization |
2-8 |
10% Mouse kidney tissue homogenization |
8-12 |
10% Mouse liver tissue homogenization |
8-12 |
10% Mouse spleen tissue homogenization |
8-12 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).