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Cat.No.:E-BC-K096-M
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Synonyms | GSH-PX |
Sample type | Serum,plasma,cells,cell culture supernatant,tissue |
Sensitivity | 17.17 U |
Detection range | 17.17-518.32 U |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 40 min |
Precision | Average inter-assay CV: 6.200%Average intra-assay CV: 2.400% |
Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
The activity of GPx was significantly reduced in the serum of patients with Keshan disease. (**P<0.01)
JC Huang et al investigate the relationship between selenium-related gene ALAD and Keshan disease. Glutathione peroxidase (GSH-Px) activity of human serum was determined using GSH-Px activity assay kit (E-BC-K096-M).
The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Mouse serum |
3-5 |
Rat serum |
5-8 |
10% Mouse brain tissue homogenate |
1 |
10% Rat liver tissue homogenate |
30-60 |
HepG2 cell homogenate (5 mgprot/mL) |
1 |
10% Plant tissue homogenate |
1-5 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Sample: serum |
PMID: 36358477 |
Sample: liver,serum |
PMID: 35489465 |
Sample: pig testicular cell (ST cell) |
PMID: 33727180 |
Sample: Tissue homogenate |
PMID: 36330218 |
Sample: liver |
PMID: 33932738 |
Sample: Tissue homogenate(kidney) |
PMID: 34399203 |
Sample: heart |
DOI: 10.3390/nu14153179 |
|
Sample: hippocampus,cortex |
DOI: 10.3390/nu14214665 |
|
Sample: serum |
Sample: serum,ruminal fluid |
PMID: 36375957 |
Sample: larvae |
PMID: 34769040 |
Sample: serum |
Sample: testicular tissue |
PMID: 36160397 |
Sample: primary cardiomyocyte |
Sample: testicular tissue |
PMID: 35443217 |
Sample: serum |
PMID: 34454183 |
Sample: brain tissue,HT-22 cell |
PMID: 34684685 |
Sample: HDF cell |
PMID: 32768965 |
Sample: Plasma,Tissue homogenate |
DOI: 10.3390/nu12113511 |
|
PMID: 33202660 |
Sample: Serum |
Sample: liver,kidney,lungs |
Sample: serum,liver |
PMID: 35819692 |
Sample: PCC 6803 strains |
DOI: 10.2147/DMSO.S366609 |
|
Sample: sciatic nerve tissue |
PMID: 35030965 |
Sample: placental tissue |
DOI: 10.1155/2021/1622270 |
|
PMID: 34409099 |
Sample: liver,kidney,cardiac |
PMID: 36241955 |
Sample: serum |
PMID: 35058758 |
Sample: hippocampus tissue |
DOI: 10.1002/jsfa.12010 |
|
PMID: 35567370 |
Sample: serum |
PMID: 32220623 |
Sample: Tissue homogenate |
DOI: 10.1111/jfbc.14306 |
|
PMID: 35766031 |
Sample: brain tissue |
DOI: 10.1111/jfbc.14397 |
|
PMID: 36069470 |
Sample: heart tissue |
DOI: 10.21037/atm-22-483 |
|
PMID: 35280364 |
Sample: myocardium |
PMID: 32438076 |
Sample: Tissue homogenate |
Sample: kidney tissue |
PMID: 28943754 |
Sample: lens tissue |
DOI: 10.2147/IJGM.S402206 |
|
Sample: Serum |
PMID: 30820147 |
Sample: lens tissue |
PMID: 32437678 |
Sample: Sperm |
Sample: Tissue homogenate(Heart) |
Sample: serum |
DOI: 10.1002/jez.2603 |
|
Sample: cortex |
DOI: 10.1111/jfbc.13865 |
|
PMID: 34263474 |
Sample: Liver,kidney |
DOI: 10.1111/jfbc.13994 |
|
PMID: 34778972 |
Sample: brain |
DOI: 10.1155/2018/7341242 |
|
Sample: kidney tissues |
Sample: heart |
Sample: follicular fluid |
PMID: 34251583 |
Sample: H9C2 cells |
DOI: 10.3390/cimb45020057 |
|
Sample: colonic tissue |
DOI: 10.1111/jfbc.13359 |
|
Sample: lung,liver,kidney |
Sample: Renal |
Sample: brain |
Sample: plasma |
M******************OSubmitted [ Oct 26 2022 ]
Asked: My i know is the formula calculation for protein is correct for Cat.No.:E-BC-K096-M ?
Reply
adminSubmitted [ Oct 26 2022 ]
Answered: Hi MUHAMMAD, I'll reply you by mail sincerely Lizzy
d*********aSubmitted [ Sep 26 2022 ]
Asked: Is the kit suitable for erythrocyte lysate?
Reply
adminSubmitted [ Sep 26 2022 ]
Answered: Dear Dragosalava, Thanks for your inquiry, we confirm with you that product #E-BC-K096-M, Glutathione Peroxidase (GSH-Px) Activity Assay Kit is suitable for erythrocyte lysate.
M*****aSubmitted [ Jul 26 2022 ]
Asked: Is the kit suitable for rabbit sample?
Reply
adminSubmitted [ Jul 26 2022 ]
Answered: Yes.
M*****aSubmitted [ Jul 26 2022 ]
Asked: Is the kit suitable for rabbit sample?
Reply
adminSubmitted [ Jul 26 2022 ]
Answered: Dear Miss.na,thanks for your message.This kit can be used with rabbit sample. Warm regards,
M*****aSubmitted [ Jul 26 2022 ]
Asked: How to determine the dilution factor of the sample?
Reply
adminSubmitted [ Jul 26 2022 ]
Answered: There is no need to dilute the sample, when the Inhibition ratio of sample is 10-50%. If the inhibition ratio is above 50%, the sample needs to be diluted.
adminSubmitted [ Jul 26 2022 ]
Answered: There is no need to dilute the sample, when the Inhibition ratio of sample is 10-50%. If the inhibition ratio is above 50%, the sample needs to be diluted.